E-ISSN 2790-2218
 

Research Article

Online Publishing Date: 14 / 11 / 2021



Molecular detection, antibiogram, and risk factor analysis of Staphylococcus aureus from subclinical mastitis of goats in conventional and organized farms

Md. Abu Zihad, Md. Ahosanul Haque Shahid, Md. Muket Mahmud, Ajran Kabir, Md. Shahriar Kamal, Jannatun Naim, Md. Liakot Hossen, KHM Nazmul Hussain Nazir.


Cited By:1

Abstract
Objectives: The goal of this study was to isolate, molecularly identify, and antibiogram of Staphylococcus aureus, as well as to investigate risk factors for subclinical mastitis in the Jhenaidah and Chuadanga districts of Bangladesh.
Materials and Methods: A total of 100 milk samples were collected from apparently healthy goats at various farms in the Jhenaidah and Chuadanga districts. To detect samples with subclinical mastitis (SCM), the California Mastitis Test (CMT) was done. S. aureus was isolated and identified from milk samples that tested positive for CMT using a combination of standard bacteriological examinations, biochemical tests, and PCR confirmation. The disc diffusion test was used to determine the antibiogram status of the isolates. The risk factors for SCM in goats were analyzed using a semi-structured questionnaire with thirteen variables.
Results: Some characteristics were significantly associated with goat mastitis, including farm, breed, shed construction, floor surface condition, and farm adviser. The CMT identified 52 positive samples for SCM, with 73.07% (n = 38/52) suggesting the presence of the nuc gene. Antibiotic susceptibility testing revealed that the isolated S. aureus was totally resistant to the antibiotics Penicillin, Oxacillin, and Vancomycin (21%). On the other hand, Tetracycline, Gentamycin, Norfloxacin, and Levofloxacin were all susceptible to all isolates.
Conclusion: SCM is a severe problem in goats in Jhenaidah and Chuadanga districts. To minimize the risk of SCM infection in goats, hygiene precautions can be followed, and education among goat farm owners and farmers can be improved.

Key words: Antibiogram; nuc gene; PCR; Subclinical mastitis; S. aureus.


 
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How to Cite this Article
Pubmed Style

Zihad MA, Shahid MAH, Mahmud MM, Kabir A, Kamal MS, Naim J, Hossen ML, Nazir KNH. Molecular detection, antibiogram, and risk factor analysis of Staphylococcus aureus from subclinical mastitis of goats in conventional and organized farms. Vet. Res. Notes. 2021; 1(2): 17-22. doi:10.5455/vrn.2021.a4


Web Style

Zihad MA, Shahid MAH, Mahmud MM, Kabir A, Kamal MS, Naim J, Hossen ML, Nazir KNH. Molecular detection, antibiogram, and risk factor analysis of Staphylococcus aureus from subclinical mastitis of goats in conventional and organized farms. https://www.vetresnotes.com/?mno=116426 [Access: February 14, 2024]. doi:10.5455/vrn.2021.a4


AMA (American Medical Association) Style

Zihad MA, Shahid MAH, Mahmud MM, Kabir A, Kamal MS, Naim J, Hossen ML, Nazir KNH. Molecular detection, antibiogram, and risk factor analysis of Staphylococcus aureus from subclinical mastitis of goats in conventional and organized farms. Vet. Res. Notes. 2021; 1(2): 17-22. doi:10.5455/vrn.2021.a4



Vancouver/ICMJE Style

Zihad MA, Shahid MAH, Mahmud MM, Kabir A, Kamal MS, Naim J, Hossen ML, Nazir KNH. Molecular detection, antibiogram, and risk factor analysis of Staphylococcus aureus from subclinical mastitis of goats in conventional and organized farms. Vet. Res. Notes. (2021), [cited February 14, 2024]; 1(2): 17-22. doi:10.5455/vrn.2021.a4



Harvard Style

Zihad, M. A., Shahid, . M. A. H., Mahmud, . M. M., Kabir, . A., Kamal, . M. S., Naim, . J., Hossen, . M. L. & Nazir, . K. N. H. (2021) Molecular detection, antibiogram, and risk factor analysis of Staphylococcus aureus from subclinical mastitis of goats in conventional and organized farms. Vet. Res. Notes, 1 (2), 17-22. doi:10.5455/vrn.2021.a4



Turabian Style

Zihad, Md. Abu, Md. Ahosanul Haque Shahid, Md. Muket Mahmud, Ajran Kabir, Md. Shahriar Kamal, Jannatun Naim, Md. Liakot Hossen, and KHM Nazmul Hussain Nazir. 2021. Molecular detection, antibiogram, and risk factor analysis of Staphylococcus aureus from subclinical mastitis of goats in conventional and organized farms. Veterinary Research Notes, 1 (2), 17-22. doi:10.5455/vrn.2021.a4



Chicago Style

Zihad, Md. Abu, Md. Ahosanul Haque Shahid, Md. Muket Mahmud, Ajran Kabir, Md. Shahriar Kamal, Jannatun Naim, Md. Liakot Hossen, and KHM Nazmul Hussain Nazir. "Molecular detection, antibiogram, and risk factor analysis of Staphylococcus aureus from subclinical mastitis of goats in conventional and organized farms." Veterinary Research Notes 1 (2021), 17-22. doi:10.5455/vrn.2021.a4



MLA (The Modern Language Association) Style

Zihad, Md. Abu, Md. Ahosanul Haque Shahid, Md. Muket Mahmud, Ajran Kabir, Md. Shahriar Kamal, Jannatun Naim, Md. Liakot Hossen, and KHM Nazmul Hussain Nazir. "Molecular detection, antibiogram, and risk factor analysis of Staphylococcus aureus from subclinical mastitis of goats in conventional and organized farms." Veterinary Research Notes 1.2 (2021), 17-22. Print. doi:10.5455/vrn.2021.a4



APA (American Psychological Association) Style

Zihad, M. A., Shahid, . M. A. H., Mahmud, . M. M., Kabir, . A., Kamal, . M. S., Naim, . J., Hossen, . M. L. & Nazir, . K. N. H. (2021) Molecular detection, antibiogram, and risk factor analysis of Staphylococcus aureus from subclinical mastitis of goats in conventional and organized farms. Veterinary Research Notes, 1 (2), 17-22. doi:10.5455/vrn.2021.a4





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